A team of researchers at Sinai Health System'sLunenfeld-Tanenbaum Research Institute (LTRI) and University of Toronto's Donnelly Centre has developed a new technology that can stitch together DNA barcodes inside a cell to simultaneously search amongst millions of protein pairs for protein interactions. The paper will be published today in the journal Molecular Systems Biology.
In recent years, DNA barcoding has enabled scientists to carry out highly parallel experiments, in which many different types of cells can be tested in the same tube. This has been further enabled by next-generation DNA sequencing which can efficiently count barcodes and 'read out' the results. However, the number of experiments that can be combined in the same tube has been limited to the number of barcoded cell types. Allowing barcodes to fuse together inside cells means scientists can now break that barrier. The new technology results in a 10-fold increased rate of discovery for the same price.




